1:1 in what way?
The quad mesh used by 2D Toolkit is built to fit the part of the sprite image that is visible. If your sprite is 32x84, the sprite will form that ratio. The physical size depends on how you set up the ortho size / wether you use tk2dCamera / etc. When you set up the sprite size in the sprite collection settings, it will appear pixel perfect at that resolution automatically without any scaling required, as long as you display it with the same camera parameters. Eg. if you build your sprite collection with "Use tk2dCamera", then display it with a tk2dCamera for it to be automatically pixel perfect.